Prokaryotic Community Determination of Metal-Rich Acidic Environment by Comparative Methods

Acid mine drainage (AMD) is a wastewater caused by mining activities and sulfide minerals oxidation. It occurs generally as result of the relation water, oxygen microorganisms. In this study, pH temperature samples were determined around 2 20 °C, respectively. The chemical analysis was made - ICP-MS. Sulfur concentration found in higher amounts to be 21,892 ppm, 6155 7893 31,305 ppm iron also high with concentrations ranging from 14,318 2181 2715 13,801 including K2, K3, K4, K5, Prokaryotic community fingerprints obtained DGGE 16S rRNA gene clone library. High-throughput sequencing fragments qPCR performed environmental DNAs. FISH carried out interacting probes. extremely acidic four ponds contained sulfate iron. Archaeal genera Acidiplasma sp. Ferroplasma abundance sulfate-rich sampling area according metagenomic approach. Acidithiobacillus, Leptospirillum Acidiphilium often acid ponds. Otherwise, Sulfobacillus sp., Ferrithrix Ferrimicrobium Acidimicrobium Aciditerrimonas at end analyses. As consequence, library, approach, combined collaboratively, complementarily comparatively for acidophilic prokaryotic diversity.